MEDUSA

MEDUSA is a simulation-assisted framework for CRISPR chemogenetic profiling that maps sgRNA fold-change data to gene-level death and growth phenotypes. Use this app to analyze treated, untreated, and baseline comparisons and prioritize candidate death-regulatory genes.

Input Files Drug treated vs untreated Untreated vs baseline (timepoint 0)

Required Parameters Untreated net population growth rate Untreated assay end time (hours) Drug treated assay end time (hours)

Optional / Advanced Parameters

Assay start time (hours) Treated growth rate before death onset* Death onset time (hours)* Treated growth rate after death onset* Treated death rate after death onset* Max guides per synthetic nontarget gene Non-targeting guide ID Guide-to-gene aggregation

General model (GM) settings

Effective dose Effect dose error Parameter space size

* if missing MEDUSA runs in general model (GM) mode and effective dose is required.

Technical simulation controls

# of simulation interations # bootstrap interations

Output Selections

Gene level summary Phase diagram Save intermediate files